Photosynthetic Assimilation of Carbon Dioxide and Acetate by Isolated Chloroplasts

Photosynthetic assimilation of carbon dioxide and acetate by isolated chloroplasts.

Chloroplasts isolated by methods similar to that described by Arnon et al. (1) are capable of incorporating acetate carbon into both lipid and n)on-lipid materials when fortified with appropriate cofactors (2, 3,4, 5). The actual rate of incorporation of acetate carbon in these preparations was, however, very small compared with the normal rate of photosynthesis (1-2 jumoles/mg chlorophyll'hr) ...

Photosynthesis by isolated chloroplasts. X. Dependence of carbon dioxide assimilation on the photochemical reactions of chloroplasts.

In addition to photosynthetic phosphorylation recent work has also provided direct experimental evidence (10-14) for the often asserted but never previously demonstrated capacity of isolated chloroplasts to assimilate CO2 photosynthetically to the level of carbohydrates. CO2 assimilation by isolated chloroplasts was found to be a dark process (15) dependent on a photochemically generated “assim...

Photosynthesis by isolated chloroplasts. Inhibition by DL-glyceraldehyde of carbon dioxide assimilation.

Photosynthetic carbon assimilation and associated CO(2)-dependent O(2) evolution by chloroplasts isolated from pea shoots and spinach leaves is almost completely inhibited by 10mm-dl-glyceraldehyde. The inhibitor is without appreciable effect on photosynthetic electron transport, photophosphorylation, the carboxylation of ribulose 1,5-diphosphate or the reduction of 3-phosphoglycerate, but appa...

Inhibition of photosynthetic carbon dioxide fixation in isolated spinach chloroplasts exposed to reduced osmotic potentials.

Reduced osmotic potentials inhibited the rate of CO(2) fixation by isolated intact spinach (Spinacia oleracea) chloroplasts. This inhibition was observed immediately after transfer of chloroplasts from a solution containing 0.33 m sorbitol to higher sorbitol concentrations, and the depressed rate remained constant. The inhibited CO(2) fixation could not be attributed to a decreased rate of phot...

Photosynthesis by Isolated Chloroplasts IX. Photosynthetic Phosphorylation and CO(2) Assimilation in Different Species.